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Composite
Fok_a-Venu

Part:BBa_K243032:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-20)

Fok_a-Venus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 487


Design Notes

The combination of the parts Fok_a and mVenus-YFP was done to gain information about protein expression. The mVenusYFP is a proven Biobrick of the [http://2007.igem.org/Freiburg team Freiburg07]. Thus, Venus fusion proteins give an easy to visualize readout of protein expression and are also good detectable by western blotting.

Designed according to the RFC 25. Commented GenBank file

Source

Combined the parts by serial cloning steps.

References